Purification and Structure Identification of Hyaluronic Acid

Polysaccharide produced by mutated strain of Streptococcus zooepidemicus was purified by the procedures including Savage method, quaternary ammonium compound precipitation, DEAE-cellulose(DE52) chromatography and Sephadex G-75 gel filtration. The structure of the purified polysaccharide has been characterized by means of chemical composition analysis, 13C NMR spectrum, infrared spectrum and circular dichroism (CD). All the results showed that the purified polysaccharide was hyaluronic acid (HA). The single helix conformation of the purified HA was determined by Congo red experiment. The molecular weight of the HA was about 1.16×106D, which was measured by viscosity method.

作 者： Shui Sheng HONG Jia Chen Jian Guo ZHANG Yan Chun TAO Lan Ying LIU   作者單位： Shui Sheng HONG,Jia Chen,Lan Ying LIU(College of Life Science,Jilin University,Changchun 130023)

Jian Guo ZHANG(State Key Laboratory of Electroanalytical Chemistry,Chinese Academy of Sciences,Changchun Institute of Applied Chemistry,Changchun 130022)

Yan Chun TAO(Key Laboratory of Supermolecular Structure and Spectroscopy of Educational Ministry,Jilin University,Changchun 130023) 

刊 名： 中國化學(xué)快報(英文版)  ISTIC SCI 英文刊名： CHINESE CHEMICAL LETTERS  年，卷(期)： 2004 15(7)  分類號：   關(guān)鍵詞： Streptococcus zooepidemicus   hyaluronic acid   structure identification  

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