Simultaneous determination of berberine, matrine and oxymatrine in traditional Chinese medicines by using nonaqueous cap

A rapid method for the simultaneous determination of berberine (BBR), matrine (MT) and oxymatrine (OMT) by nonaqueous capillary electrophoresis (NACE) was developed. Optimum separation of the analytes was obtained on a 50?cm×50?μm i.d. fused-silica capillary using a non-aqueous buffer system of 70?mM ammonium acetate, 7.0% acetic acid and 10% acetonitrile at 25?kV and 20?℃. The relative standard deviations (R.S.D.) of the migration times and peak areas of the three active components were 0.06%-0.20% and 0.12%-3.41% for berberine, 0.11%-0.60% and 0.74%-1.63% for matrine, 0.15% and 0.45% for oxymatrine, respectively. Detection limits of berberine, matrine and oxymtrine were 0.18?μg/mL, 4.08?μg/mL and 4.16?μg/mL, respectively. In the tested concentration range, good linear relationships (0.999?2 for berberine, 0.998?8 for matrine and 0.998?8 for oxymatrine) were observed. The linear calibration ranges were 0.45-360.0?μg/mL for berberine, 8.16-408.0?μg/mL for matrine and 20.8-416.0?μg/mL for oxymatrine. This method has been successfully applied to the phytochemical analysis of alkaloids extracts from two commonly used traditional Chinese herbal drugs: Sophora flavescens Ait. (Kushen) and Cortex phellodendri chinensis (Huangbai) and their medicinal preparations.

作 者： Yan-Hua Li Zhuo Zhang Wen Lu Lang-Chong He   作者單位： School of Medicine, Xi'an Jiaotong University, Xi'an 710061, P.R.China  刊 名： 西安交通大學(xué)學(xué)報(bào)（英文版）  英文刊名： ACADEMIC JOURNAL OF XI'AN JIAOTONG UNIVERSITY(ENGLISH EDITION)  年，卷(期)： 2010 22(1)  分類號(hào)： U4  關(guān)鍵詞： berberine   matrine   oxymatrine   nonaqueous capillary electrophoresis   Sophora flavescens Ait   Cortex phellodendri chinensis  

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